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The application of exogenous melatonin prompted the endogenous melatonin biosynthetic process through antagonistic interaction with calcium, thereby safeguarding the product against chilling injury and postharvest deterioration, as indicated in Figure 5B

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For Target B samples that were biotinylated in vivo, a BirA expressing plasmid was co-transfected and media was supplemented with sterile filtered biotin that was resuspended in OptiMEM media (Gibco, ThermoFisher Catalog Number: 31985070)